However, AST68 was selected for a molecular-docking analysis using AutoDOCK Vina and AutoDOCK 4.2 with the generated 3D model, showing that both domains were well superimposed on the homologs. The AST68 and ABCG40 potential GSL TPs were chosen for further investigation because their subcellular localisation is similar to that of known GSL TPs (SULTR1 1 and SULTR1 2) and ABCG36, respectively. Based on three criteria, 21 potential GSL genes encoding TPs were selected. In addition, 1151 co-expressed genes were annotated, integrated, and visualised using relevant bioinformatic tools. thaliana GSL genes were retrieved from the literature and pathway databases and searched against several co-expression databases to generate a gene network consisting of 1267 nodes and 14,308 edges. This study aimed to identify potential GSL-transporter proteins (TPs) using a network-biology approach. thaliana, the reallocation of defence compounds, of which GSLs are a major part, is highly dependent on transport processes and serves to protect high-value tissues such as reproductive tissues. Several species in Brassicaceae produce glucosinolates (GSLs) to protect themselves against pests.
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